Guanidine hydrochloride, denaturant, chaotrope A selection of strong chemicals to aid in the denaturation of proteins Denaturants, or chaotropes, disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptidesGuanidineHCl, Molecular Grade, is commonly used for the isolation of intact mRNA from tissues or cultured cells Formula Weight 9553 Form Fine, colorless or white crystals Properties Purity ≥995% A 230 at 6M ≤015 A 260 at 6M ≤003 A 280 at 6M ≤002 Moisture ≤03% Melting Point 186–1°C Lead ≤5ppm Zinc ≤1ppm Copper ≤1ppmAfter centrifugation, the pellet is washed with buffer containing either low concentrations of chaotropic agents (eg 051 M guanidineHCl or urea) or
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Guanidine hcl sds-Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo It is a potent denaturant and inactivator of several enzymes and proteins塩化グアニジニウム（塩酸グアニジニウム、英： Guanidinium chloride ）とは、グアニジンの塩酸塩。 GdmClやGndCl、GuHClと略されることがある。主にタンパク質 変性剤として利用される。
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Guanidine Hydrochloride Section 1 Identification 11 Product Identifiers Product Name Guanidine Hydrochloride CAS# RTECS# MF Synonyms Guanidine Monohydrochloride, Guanidinium Chloride, Guanidinium Hydrochloride 12 Recommended Use of the Chemical and restrictions of Use Chemical manufacturing 13 Supplier Details BioSpectra1Methylguanidine hydrochloride C2H8ClN3 CID structure, chemical names, physical and chemical properties, classification, patents, literature, biologicalGuanidine13C hydrochloride DTXSID (~13~C)Guanidinehydrogen chloride (1/1) Guanidine13C hydrochloride, 99 atom % 13C
Guanidine Hydrochloride 6M solution can be used to denature proteins which become randomly coiled with no residual structure This concentration is also used to decrease enzyme activity The denatured proteins can be modified or analyzed using mass spectrometry, electrophoresis and enzymatic digestsBuy GE1914 Guanidine hydrochloride, 99% () online from Glentham Life Sciences, a manufacturer and supplier of fine chemicals View catalogue prices, chemical data, technical specifications and MSDS documentsGuanidine hydrochloride, PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production, Guanidine hydrochloride, BioUltra, for molecular biology, ≥995% (AT),
Synonym 2Guanidine4methylquinazoline hydrochloride, 4Methyl2guanidino quinazoline hydrochloride, N(4Methyl2quinazolinyl)guanidine hydrochloride, NSC hydrochloride Empirical Formula (Hill Notation) C 10 H 11 N 5 · HClGuanidine Hydrochloride, BiotechGrade is considered a chaotrope and a strong denaturants utilized in physiochemical studies of protein folding Proteins become randomly coiled at high concentrations of guanidinium chloride Spectrum offers highly pure reagents suitable for biochemical research and analysisSep 14, · Note This document contains side effect information about guanidine Some of the dosage forms listed on this page may not apply to the brand name Guanidine Hydrochloride Applies to guanidine oral tablet Side effects requiring immediate medical attention Along with its needed effects, guanidine (the active ingredient contained in Guanidine Hydrochloride) may
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Guanidine hydrochloride (CDXG0040), CAS , is a high purity chemical used as powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids It is useful for denaturation and refolding of proteins Purification of proteins and nucleic acids, refolding of denatured proteinsGuanidine hydrochloride has also typically been used for the isolation of RNA, to denature globular proteins, and for protein refolding studies It can also be used to facilitate the generation of tryptic peptides for analysis of complex protein samplesThe Approved Drug Products with Therapeutic Equivalence Evaluations List identifies currently marketed prescription drug products, incl guanidine hydrochloride, approved on the basis of safety and effectiveness by FDA under sections 505 of the Federal Food, Drug, and Cosmetic Act /Guanidine Hydrochloride/
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Abstract The activity and conformational change of human placental cystatin (HPC), a low molecular weight thiol proteinase inhibitor (12,500) has been investigated in presence of guanidine hydrochloride (GdnHCl) and urea The denaturation of HPC was followed by activity measurements, fluorescence spectroscopy and Circular Dichroism (CD) studiesAminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride Guanidine hydrochloride, >=99% (titration), organic base and chaeotropic agent Guanidine hydrochloride, PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production 3 Chemical and Physical PropertiesFind patient medical information for guanidine oral on WebMD including its uses, side effects and safety, interactions, pictures, warnings and user ratings
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Guanidine is a small, nitrogenrich organic compound found in nature in plants (eg, rice hulls and turnip juice) and animals (eg, mussels and earthworms) Unlike its oxygen analogue, carbonic acid, it exists at ambient conditions (ie, not in solution nor at cryogenic temperatures)Guanidine HCl (Aminoformamidine), the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant In vitro Guanidine HCl is the most popular protein denaturant Analysis of unfolding transitions by Guanidine HCl provides several important parameters regardingGuanidine Hydrochloride Product Code(s) Revision Date 08Aug11 MSDS M110 Target Organ Effects Bone marrow, Nerves Product Information Harmful if swallowed, inhaled, or absorbed through skin Causes skin, eye and respiratory tract irritation 10 STABILITY AND REACTIVITY Hazardous Polymerization Hazardous polymerization does not occur
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Guanidinium chloride (also known as guanidine hydrochloride) has chaotropic properties and is used to denature proteins Guanidinium chloride is known to denature proteins with a linear relationship between concentration and free energy of unfoldingGuanidine Hydrochloride is a strong protein denaturant that functions as a chaotropic agent As a denaturant, it acts to unfold proteins and turn them into their original polypeptide chains As a chaotropic agent, it breaks down the structure of proteins Guanidine Hydrochloride is commonly used in the purification of RNA by dissociating theGuanidine Hydrochloride was used to decrease enzyme activity of streptomycin adenylyltransferase and increase the solubility of hydrophobic molecules At high concentrations of guanidinium chloride (eg, 6 M), proteins lose their ordered structure, and they tend to become randomly coiled, ie they do not contain any residual structure
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Guanidine and its derivatives are used in manufacture of plastics, resins, photochemicals, fungicides, rubber chemicals, and disinfectants Guanidine hydrochloride, the hydrochloride salt of guanidine, is commercially available It is a common chemical intermediate in manufacturing, as noted above It has been used to produce nitroguanidine, aAccording to the manufacturer, guanidine is excreted in breast milk and breastfeeding should be discontinued during treatment Due to a lack of data evaluating safety in the nursing infant, and the potential for hematologic, neurologic, and cardiac toxicity associated with the drug, breastfeeding should be avoided if guanidine therapy is necessary in the postpartum motherThermo Scientific Pierce GuanidineHCl can be easily dissolved and pHadjusted to any concentrationFeatures of GuanidineHCl No wasting valuable research time weighing and dissolving guanidine crystals Free from UV absorbing materials in the range of nm Free from heavy metal contaminants Fr
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Guanadine HCL is one of the strongest denaturing agents for protein folding studies, and is used to denature proteins due to its chaotropic qualitiesGuanidine HCL is a chaotropic salt Chaotropic salts are critical for cell lysis and binding to the silica resin Specifically, Chaotropes have two important roles in nucleic acid extraction Destabilize hydrogen bonds, van der Waals forces and hydrophobic interactions Leading to destabilization of proteins (including nucleases)Guanidine Hydrochloride CAS Calbiochem CAS Guanidine HCL is a powerful protein denaturant Find MSDS or SDS, a COA, data sheets and more information
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Oct 03, 17 · Guanidine Hydrochloride is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids, especially mRNA Guanidine Hydrochloride is useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins and isolation of RNAProtein Denaturation Urea vs Guanidine Hydrochloride (GdnHCl) In equilibrium denaturation experiments, the concentration of urea at midpoint of denaturation is always higher than that ofShop a large selection of Carboximidamides products and learn more about Guanidine hydrochloride, 98%, with 04% anticaking agent (SiO2), ACROS Organics 250g;
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Guanidine hydrochloride (GdnHCl) is hydroscopic and a small molecule It is a potent denaturant of enzymes, which results in conformational changes in the enzyme, leading to complete loss of enzyme activity GdnHCl is involved in blocking the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivoGuanidine Hydrochloride is a strong protein denaturant that functions as a chaotropic agent As a denaturant, it acts to unfold proteins and turn them into their original polypeptide chains As a chaotropic agent, it breaks down the structure of proteinsThe guanidine hcl available here can be used in a variety of distinct pharmaceutical, organic and other chemical processes Leading suppliers and wholesalers offer these highquality items on the site for affordable prices and discounts
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CAS MDL MFCD EINECS Synonyms Guanidinium chloride, Guanidine hydrochloride, Guanadine hydrochloride, Guanidine chloride Suited for purification of nucleic acids or polypeptides from cell sources and inhibition of both RNase and protease activityUse of the information, documents and data from the ECHA website is subject to the terms and conditions of this Legal Notice, and subject to other binding limitations provided for under applicable law, the information, documents and data made available on the ECHA website may be reproduced, distributed and/or used, totally or in part, for noncommercial purposes providedDescription Thermo Scientific Pierce 8M GuanidineHCl Solution is an accurately formulated, readytouse solution of guanidine hydrochloride, that can be easily diluted and pHadjusted to any concentration below 8M Features of 8M GuanidineHCl Solution • Any concentration below 8M guanidine can be quickly and easily prepared
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Aug 10, · DESCRIPTION Chemically, guanidine (aminomethanamidine) hydrochloride is a crystalline powder freely soluble in water and alcohol The aqueous solution is neutral The structural formula is Each tabletGuanidine HCl, the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant;Target Guanidine HCl is the most popular protein denaturant Analysis of unfolding transitions by Guanidine HCl provides several important parameters regarding the mechanism ofGuanidine hydrochloride is commercially available from Sigma Aldrich The MW is 955 therefore one mole weight 955 g If you want to prepare one liter of solution you need 8 x 955 g of this salt
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GUANIDINE HYDROCHLORIDE Tablets DESCRIPTION Chemically, guanidine (aminomethanamidine) hydrochloride is a crystalline powder freely soluble in water and alcohol The aqueous solution is neutral The structural formula is Each tablet contains 125 mg of guanidine hydrochloride with no color additive in the baseGuanidine HCl may agglomerate upon storage It may appear as a freeflowing crystalline powder, a free flowing powder with solid material dispersed throughout, or a solid The quality of the product does not appear to be affected and solutions prepared from the freeflowing and lumpy guanidine HCl
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